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PHISTO is a bioinformatics Web platform for studying infection mechanisms through Pathogen-Host Interactions (PHIs). PHISTO enables access to the most up-to-date PHI data for all pathogen types for which experimentally verified protein interactions with human are available. Currently storing 23663 protein-protein interactions between 301 pathogenic strains and human, PHISTO aims to provide the entirety of relevant information about PHIs. Moreover, it includes  bioinformatics tools to analyze PHI networks through a user-friendly and functional Web interface at PHISTO offers bioinformatics tools for visualization of PHI networks as protein interaction graphs, graph-theoretical analysis of pathogen-targeted human proteins and BLAST search for orthologous PHI data for pathogens which have no experimental data. Among the computational tools provided by PHISTO web platform are GO and Pathway enrichment analysis of selected proteins.