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PHI-Seq is a bioinformatic analysis platform for the analysis of many sequence data obtained by the Next Generation Sequencing (NGS) method, which is being developed within the scope of ongoing R&D activities. There is a need for tools that enable efficient analysis of transcriptomic (RNA sequence) data obtained by NGS. Within the scope of this project, it is aimed that these tools will be developed on the PHI-Seq platform, which will be commercialized by creating a user-friendly interface, and will enable the analysis of RNA-Seq data. NGS technique diagnostic, academic, agricultural and so on. The challenge of analyzing RNA-Seq data reveals new software needs around the world. With an efficient, useful and functional software that will allow easy analysis. It is aimed to create an answer to the need.